Caracterización inmunofenotípica del Colangiocarcinoma. Revisión de 67 casos diagnosticados en el laboratorio de Patología Contreras Robledo en los últimos 5 años.

Franchely Rosario P.; Isabel Fernández G.; Carolin Peña M.; Salma   Sánchez F.; Nelsin Rodríguez L.; Alexis   Flaquer F.; Félix Contreras M.

Authors

Franchely Rosario P. Laboratorio de Patología Contreras Robledo, Santiago de los Caballeros, Dominican Republic and Faculty of Health Sciences, Facultad de Ciencias de la salud Pontificia Universidad Católica Madre y Maestra
Isabel Fernández G. Laboratorio de Patología Contreras Robledo, Santiago de los Caballeros, Dominican Republic and Faculty of Health Sciences, Facultad de Ciencias de la salud Pontificia Universidad Católica Madre y Maestra
Carolin Peña M. Internal Medicine Resident, Clínica Universitaria Unión Médica, Santiago de los Caballeros, Dominican Republic
Salma Sánchez F. Internal Medicine Resident, Clínica Universitaria Unión Médica, Santiago de los Caballeros, Dominican Republic
Nelsin Rodríguez L. Internal Medicine Resident, Clínica Universitaria Unión Médica, Santiago de los Caballeros, Dominican Republic
Alexis Flaquer F. Interventional Radiologist and Internist, Clínica Universitaria Unión Médica, Santiago de los Caballeros, Dominican Republic
Félix Contreras M. Laboratorio de Patología Contreras Robledo, Santiago de los Caballeros, Dominican Republic and Faculty of Health Sciences, Facultad de Ciencias de la salud Pontificia Universidad Católica Madre y Maestra

Keywords:

cholangiocarcinoma, immunohistochemistry, markers, CK7, CK20, mucins

Abstract

Cholangiocarcinoma (CCA) is a malignant neoplasm of the biliary epithelium whose differential diagnosis from other primary hepatic neoplasms and hepatic metastases is often challenging. In this context, immunophenotypic analysis is an essential tool for its characterization. Objectives: Characterize the immunohistochemical profile of cholangiocarcinomas diagnosed at the Contreras Robledo Pathology Laboratory over the past five years. Methods: A descriptive, retrospective study was conducted between January 2020 and August 2025. Demographic and morphological variables, as well as immunophenotypic findings, were evaluated. Results: A total of 67 cases were identified, with a female predominance (61.1%) and a mean age of 61 years (range: 16-89). The CK19 marker was positive in all evaluated cases. The most frequent immunophenotype was CK7+/CK20-, observed in 92.8% of cases. Hepatic markers Hep-Par1 and Arginase-1 were negative in 95% of cases. Conclusions: CCA exhibits a characteristic immunophenotypic profile. The combination of CK7+/CK20- and the absence of hepatocellular markers and/or other metastatic markers is useful for the differential diagnosis from other hepatic neoplasms and metastases from other primary tumors.

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